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目的:研究人肝癌细胞株HepG2对同时包载齐墩果酸(OA)和香豆素6的乳酸羟基乙酸共聚物纳米粒(OCPN)和乳酸羟基乙酸共聚物-水溶性维生素E纳米粒(OCPTN)的体外摄取情况。方法:以超声乳化-溶剂挥发法制备OCPTN和OCPN,其中香豆素6为荧光标记物;采用高效液相色谱法测定两种纳米粒中香豆素6的载药量,体外测定HepG2细胞对分别含100、200、400μg/ml(低、中、高质量浓度)香豆素6的OCPTN、OCPN混悬液的摄取率,显微镜观察HepG2细胞对OCPTN的摄取情况。结果:OCPTN和OPTN中香豆素6的载药量分别为7.6%、6.3%;低、中、高质量浓度OCPTN的细胞摄取率为(62.1±1.2)%、(53.6±1.3)%、(40.9±1.5)%,分别是相同质量浓度OCPN的细胞摄取率[(36.8±1.5)%、(31.2±1.9)%、(22.4±1.3)%]的1.69、1.72、1.83倍;镜下观察OCPTN被HepG2细胞摄取,处于细胞核周围。结论:OCPN和OCPTN均能被HepG2细胞摄取,且OCPTN的被摄取性更强。
OBJECTIVE: To investigate the effect of HepG2 on the activity of OCPN and OCPTN on the co-encapsulation of oleanolic acid (OA) and coumarin 6 nanoparticles (OCPN) and lactic acid glycolic acid In vitro intake. Methods: OCPTN and OCPN were prepared by ultrasonic emulsification-solvent evaporation method, of which Coumarin 6 was a fluorescent marker. The drug loading of coumarin 6 in the two kinds of nanoparticles was determined by high performance liquid chromatography (HPLC) The uptake rates of OCPTN and OCPN suspension containing 100, 200, 400 μg / ml Coumarin 6 (low, medium and high concentration), and OCPTN uptake were observed under a microscope. Results: The drug loading rates of coumarin 6 in OCPTN and OPTN were 7.6% and 6.3%, respectively. The uptake rates of OCPTN in low, middle and high concentrations were (62.1 ± 1.2)% and (53.6 ± 1.3)%, respectively 40.9 ± 1.5)%, respectively, which was 1.69,1.72 and 1.83 times higher than those in the same mass concentration of OCPN [(36.8 ± 1.5)%, (31.2 ± 1.9)%, (22.4 ± 1.3)%] Ingested by HepG2 cells, around the nucleus. Conclusion: Both OCPN and OCPTN can be taken up by HepG2 cells, and OCPTN is more ingested.