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目的研究磁性纳米四氧化三铁颗粒(Fe3O4-MNPs)的制备及比较不同浓度的Fe3O4-MNPs对ICR小鼠脏器的影响。方法化学共沉淀法制备Fe3O4-MNPs;透射电镜、扫描电镜观察Fe3O4-MNPs的形貌;不同剂量Fe3O4-MNPs(0、300、600、1200mg/kg)给40只小鼠分4组(每组10只)一次性喂食,14d后处死小鼠收集标本,取小鼠皮肤、肝脏、脾脏、大肠,观察其组织病理学改变。结果化学共沉淀法制备出黑色颗粒状晶体。透射电镜观察发现Fe3O4-MNPs外形呈现大小不等的近似球形,扫描电镜观察发现Fe3O4-MNPs外形呈现大小不等的不规则状。不同剂量Fe3O4-MNPs喂食的ICR小鼠皮肤、肝脏、大肠及脾脏组织病理学均无明显差异。结论化学共沉淀法成功制备了Fe3O4-MNPs。一定剂量范围的Fe3O4-MNPs对正常小鼠脏器无明显的组织病理学损害。
Objective To study the preparation of magnetic nano-Fe3O4-MNPs and compare the effect of different concentrations of Fe3O4-MNPs on the organs of ICR mice. Methods Fe3O4-MNPs were prepared by chemical coprecipitation method. The morphology of Fe3O4-MNPs was observed by transmission electron microscopy and scanning electron microscopy. Forty mice were divided into 4 groups (0, 300, 600 and 1200 mg / kg) 10) were fed at one time. After 14 days, the mice were sacrificed and the mice were sacrificed. The skin, liver, spleen and large intestine of mice were collected and their histopathological changes were observed. Results Chemical coprecipitation method to prepare black granular crystal. The shape of Fe3O4-MNPs was observed by TEM. The shape of Fe3O4-MNPs was approximately spherical with different sizes. Scanning electron microscopy showed that the shape of Fe3O4-MNPs was irregular in shape. The histopathology of skin, liver, colon and spleen in ICR mice fed different doses of Fe3O4-MNPs showed no significant difference. Conclusion Chemical coprecipitation successfully prepared Fe3O4-MNPs. A certain dose range of Fe3O4-MNPs had no significant histopathological damage to normal mouse organs.