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细胞培养是一种较新的研究技术。近二十年来,在研究粉尘的致病作用中已广泛地采用细胞培养,并把这一方法作为粉尘研究中的一个重要手段。在文献报道中,用动物的腹腔巨噬细胞较多,粉尘多为矽尘。但在尘肺的发病机理中,肺巨噬细胞(AM)却占有重要的位置。为了研究游离SiO_2含量不同的粉尘对AM的毒性,我们用细胞培养方法,以细胞着色率、存活率为指标,观察了六种粉尘对AM的毒性。
Cell culture is a relatively new research technique. In the past two decades, cell culture has been widely used in the study of the pathogenic role of dust, and this method has been used as an important tool in dust research. Reported in the literature, the use of animal peritoneal macrophages more dust mostly silica dust. However, in the pathogenesis of pneumoconiosis, pulmonary macrophages (AM) occupy an important position. In order to study the toxicity of different dusts with different content of SiO 2 to AM, we used cell culture method to evaluate the cytotoxicity of six kinds of dust on AM by using cell color and survival rate as indexes.