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目的探讨缩宫素对大鼠肝脏缺血-再灌注(I-R)损伤的保护作用及可能的机制。方法将48只雌性SD大鼠随机均分为假手术(S)组、I-R组和缩宫素处理(OT)组。建立大鼠70%I-R模型,缺血时间1 h。OT组分别于术前12 h,15 min及再灌注时经腹腔注射缩宫素0.5 mg/kg,S组及I-R组在相同时间注射等量生理盐水。各组大鼠分别于肝脏再灌注后2和6 h处死,取肝脏及血液标本,检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、肿瘤坏死因子α(TNF-α)以及肝脏组织超氧化物歧化酶(SOD)、髓过氧化物酶(MPO)的活性及丙二醛(MDA)含量,并分别检测肝组织中核因子κB(NF-κB)的表达和肝脏组织的病理改变。结果与I-R组相比,OT组的ALT、AST、TNF-α水平及MPO、NF-κB阳性表达明显降低(P<0.05),肝脏病理损伤较轻,但是MDA及SOD变化不明显。结论缩宫素对大鼠肝脏I-R损伤具有保护作用。其机制可能与抑制炎症因子产生及活化有关。
Objective To investigate the protective effect of oxytocin on liver ischemia-reperfusion (I-R) injury in rats and its possible mechanism. Methods 48 female Sprague-Dawley rats were randomly divided into sham operation (S) group, I-R group and oxytocin treatment group (OT). Establishment of 70% I-R rat model, ischemic time 1 h. OT group was injected intraperitoneally with oxytocin 0.5 mg / kg at 12 h, 15 min and reperfusion respectively. S and I-R groups were injected with the same volume of saline at the same time. The rats in each group were sacrificed at 2 and 6 h after liver reperfusion, and the contents of serum ALT, AST, TNF-α, (SOD), myeloperoxidase (MPO) and malondialdehyde (MDA) were measured. The expression of NF-κB and the pathological changes of liver tissue were detected. Results Compared with I-R group, the levels of ALT, AST, TNF-α, MPO and NF-κB in OT group were significantly lower than those in I-R group (P <0.05). Conclusion Oxytocin has a protective effect on rat liver I-R injury. The mechanism may be related to inhibiting the production and activation of inflammatory factors.