论文部分内容阅读
甲状旁腺素（ＰＴＨ）是调节钙磷代谢的经典激素，有报道ＰＴＨ对其靶细胞－成骨细胞有促增殖分化作用。经多层次、多水平的实验研究证实，ＰＴＨ对成骨样细胞ＲＯＳ１７／２．８确有促增殖作用。（１）细胞计数、ＭＴＴ［３－（４，５－ｄｉｍｅｔｈｙｌｔｈｉａ－ｚｏｌ－ｚ－ｙｉ）２，５－ｄｉｐｈｅｎｙｌｔｅｔｒａｚｏｌｉｕｍｂｒｏｍｉｄｅ］测定及ＳＲＢ（ｓｏｄｉｕｍｒｈｏｄａｍｉｎｅＢ，ＳＲＢ）染色均显示经ＰＴＨ（１０－９ｍｏｌ／Ｌ）处理的细胞，其数目明显增加；（２）３Ｈ－ＴｄＲ参入增加；（３）与增殖相关的原癌基因（ｃ－ｆｏｓ、ｃ－ｊｕｎ、ｃ－ｋｉ－ｒａｓ和ｃ－ｍｙｃ）的表达增强；（４）成骨细胞特征性蛋白－碱性磷酸酶活性降低．这些结果不仅表明该激素具有非经典样作用，同时意味着激素也参与其靶细胞增殖分化的调节作用
Parathyroid hormone (PTH) is a classical hormone regulating calcium and phosphorus metabolism, PTH has been reported on its target cells - osteoblast proliferation and differentiation. The multi-level, multi-level experimental studies have confirmed that PTH osteoblast-like cells ROS17 / 2.8 does have a role in promoting proliferation. (1) Cell count, MTT assay and SRB staining showed that PTH (10-9mol / L) (2) 3H-TdR incorporation increased; (3) the expression of proto-oncogenes (c-fos, c-jun, c-ki-ras and c-myc) (4) osteoblasts characteristic protein - alkaline phosphatase activity decreased. These results not only indicate that the hormone has a non-canonical effect, but also imply that hormones are also involved in the regulation of the proliferation and differentiation of their target cells