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目的 :探讨转化生长因子β1(transforminggrowthfactorβ1,TGFβ1)及其Ⅰ、Ⅱ型受体TGFβR Ⅰ、TGFβR Ⅱ与子宫内膜癌发生及临床病理参数的关系。 方法 :采用免疫组化多克隆抗体技术检测 30份正常子宫内膜癌组织、15份子宫内膜复合增生组织 (其中 9份有不典型增生 )、12份正常子宫内膜组织中TGFβ1及其受体TGFβR Ⅰ、TGFβR Ⅱ的表达。结果 :子宫内膜癌及复合增生组织中TGFβ1表达明显高于正常子宫内膜组织中者 (P<0 .0 1,P <0 .0 5) ,而前两者之间差异无显著性 (P >0 .0 5) ,且TGFβ1表达水平与子宫内膜癌肌层浸润深度呈正相关 (P <0 .0 5)。从正常子宫内膜、复合增生到子宫内膜癌组织中TGFβR Ⅰ、TGFβR Ⅱ表达逐渐下降甚至缺如 ,且两两之间差异均有显著性 (P <0 .0 5)。TGFβR Ⅰ、TGFβR Ⅱ均与肌层浸润深度呈负相关 (P <0 .0 5)。 结论 :TGFβ1过度表达可能促进子宫内膜癌的进展 ,可能是子宫内膜癌发生的早期现象 ,其负性调控的丧失与TGFβR Ⅰ、TGFβR Ⅱ表达下降或缺如有关。
Objective: To investigate the relationship between transforming growth factor β1 (TGFβ1), TGFβR Ⅰ, TGFβR Ⅱ and the occurrence and clinicopathological parameters of endometrial carcinoma. Methods: Immunohistochemical polyclonal antibody was used to detect the expression of TGFβ1 and its receptor in 30 normal endometrial cancer tissues, 15 endometrial hyperplasia tissues (9 of which were dysplasia) and 12 normal endometrial tissues Expression of TGFβR Ⅰ and TGFβR Ⅱ. Results: The expression of TGFβ1 in endometrial carcinoma and compound hyperplasia was significantly higher than that in normal endometrium (P <0.01, P <0.05), but there was no significant difference between the two P> 0.05), and the expression of TGFβ1 was positively correlated with the depth of myometrial invasion (P <0.05). The expression of TGFβR Ⅰ and TGFβR Ⅱ gradually decreased or even disappeared from normal endometrium to complex endometrial carcinoma, and the difference between any two groups was significant (P <0.05). TGFβR Ⅰ, TGFβR Ⅱ were negatively correlated with the depth of myometrial invasion (P <0.05). CONCLUSION: Overexpression of TGFβ1 may promote the progression of endometrial carcinoma and may be an early phenomenon of endometrial carcinoma. The loss of negative regulation of TGFβ1 may be associated with the decrease or absence of TGFβR Ⅰ and TGFβR Ⅱ.