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乙型肝炎病毒感染者的精子与去透明带金黄地鼠卵受精制备染色体，并以ＨＢＶ 全长ＤＮＡ 作探针对精子染色体进行荧光原位杂交。结果发现慢性迁延型肝炎患者、慢性活动型肝炎患者和ＨＢＶ 携带者的精子染色体畸变率分别为１２－３３ ％ （９／７３） 、１６－１８％ （１１／６８） 和１５－４８ ％（１３／４８），均显著高于健康对照者精子畸变率（４ ．３５％ ，５／１１５） 。进一步ＦＩＳＨ 显示，在１ 例慢性迁延型肝炎患者的精子染色体上有特异荧光信号。研究结果表明，ＨＢＶ 能导致精子染色体不稳定性，并在患者精子染色体上整合，其整合特点是：整合位点是随机的，且大多为多位点整合。
Chromosomes were prepared by fertilizing sperm of Hepatitis B virus-infected and golden-hamster de-zona pellucida, and the full-length HBV DNA was used as a probe to detect the sperm chromosome by fluorescence in situ hybridization. The results showed that in patients with chronic persistent hepatitis, the rates of chromosome aberration in patients with chronic active hepatitis and HBV carriers were 12-33% (9/73), 16-18% (11/68) and 15-48% (13%), respectively / 48), which were significantly higher than that of healthy controls (4.35%, 5/115). Further FISH showed that there was a specific fluorescence signal in the sperm chromosome of one patient with chronic persistent hepatitis. The results show that HBV can lead to chromosomal instability of sperm, and the integration of the patient’s sperm chromosome, the integration features are: integration sites are random, and mostly multi-site integration.