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By adsorbing chitosan(CS)-functionalized Prussian blue(PB) nanoparticles(CS/PB NPs) complexing DNA onto the surface of gas encapsulated microbubbles(MBs), a multifunctional gene delivery system of MBs@CS/PB/DNA was fabricated for photothermally enhanced gene transfection through ultrasound-targeted microbubble destruction. CS/PB NPs of(2.69 ± 0.49) nm could complex DNA effectively when the mass ratio was2:1. It was found that MBs@CS/PB/DNA could enhance ultrasound imaging greatly both in vitro and in vivo. In addition, MBs@CS/PB/DNA could be disrupted by applying a higher-intensity ultrasound irradiation to release CS/PB/DNA, which could effectively transform the nearinfrared(NIR) light into heat to assist the uptake of CS/PB/DNA by cells. With the aid of ultrasound irradiation and NIR light irradiation, the gene transfection efficiency was significantly enhanced to(43.08 ± 1.13) %, much higher than polyethylenimine. Moreover, MBs@CS/PB/DNA showed excellent biocompatibility, encouraging the further exploration of MBs@CS/PB/DNA to be a platform for combined ultrasound image, photothermal therapy, drug delivery, and gene therapy.
By adsorbing chitosan (CS) -functionalized Prussian blue (PB) nanoparticles (CS / PB NPs) complexing DNA onto the surface of gas encapsulated microbubbles (MBs), a multifunctional gene delivery system of MBs @ CS / PB / DNA was fabricated for photothermally Enhanced gene transfection through ultrasound-targeted microbubble destruction. CS / PB NPs of (2.69 ± 0.49) nm could have been successfully used in the mass ratio was2: 1. It was found that MBs @ CS / PB / DNA could enhance ultrasound imaging maxim both In addition, MBs @ CS / PB / DNA could be disrupted by applying a higher-intensity ultrasound irradiation to release CS / PB / DNA, which could effectively transform the near infrared (NIR) light into heat to assist the uptake of CS / PB / DNA by cells. With the aid of ultrasound irradiation and NIR light irradiation, the gene transfection efficiency was significantly enhanced (43.08 ± 1.13)%, much higher than polyethylenimine. showed excellent biocompatibility, encourag ing the further exploration of MBs @ CS / PB / DNA to be a platform for combined ultrasound image, photothermal therapy, drug delivery, and gene therapy.