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目的:观察不同剂量红花注射液对兔脊髓慢性损伤后的治疗作用。方法:将60只健康青紫蓝家兔随机分为低剂量组(A组)、中剂量组(B组)、高剂量组(C组)、盐水对照组(D组),正常饲养1个月,采用螺钉植入法建立慢性脊髓损伤动物模型。造模成功后,实验组各组分别通过耳缘静脉注射不同剂量的红花注射液,连续注射2周,对照组注射生理盐水。治疗后每组分别于术前1个月、术后6个月、给药后2周随机抽取5只家兔,以伤处为中心取出1.0cm的脊髓组织,通过免疫组化进行MMP-2检测。同时对每组随机抽取的5只家兔进行心脏采血,通过ELISA法检测血清中MMP-12的表达变化,并于不同时间点分别通过斜板试验、改良Tarlov评分评价家兔行为学变化。结果:1)各组在造模后MMP-2,MMP-12的表达以及行为学评价较造模前比较,差异有统计学意义(P<0.05)。2)给药后A,B,C组MMP-2,MMP-12表达及行为学评价较D组比较,差异有统计学意义(P<0.05),中剂量组MMP-2,MMP-12的表达以及行为学评价较高,与低剂量组比较差异有统计学意义(P<0.05)。结论:中剂量红花注射液对治疗脊髓慢性损伤较其它剂量效果显著提高,此剂量可作为红花注射液治疗脊髓慢性损伤的参考剂量。
Objective: To observe the therapeutic effects of different doses of safflower injection on chronic spinal cord injury in rabbits. Methods: Sixty healthy blue-purple blue rabbits were randomly divided into low dose group (A group), middle dose group (B group), high dose group (C group) and saline control group (D group) , The animal model of chronic spinal cord injury was established by screw implantation. After successful modeling, the experimental groups were injected with different doses of safflower injection through the ear marginal veins for two weeks, respectively. The control group was given normal saline. After treatment, each group were randomly divided into 5 groups: 1 month preoperatively, 6 months postoperatively, and 2 weeks post-administration. Spinal cord tissues of 1.0 cm were removed from the wounds and MMP-2 Detection. At the same time, 5 randomly selected rabbits in each group were subjected to cardiac blood sampling, and the changes of serum MMP-12 expression were detected by ELISA. Tarlov score was modified by oblique plate test at different time points to evaluate the changes of rabbit behavior. Results: 1) The expression of MMP-2 and MMP-12 and the behavioral evaluation of each group after modeling were significantly higher than those before modeling (P <0.05). 2) Compared with group D, the expression of MMP-2 and MMP-12 in group A, B and C after administration was significantly lower than that in group D (P <0.05); The expression of MMP-2 and MMP- Expression and behavioral evaluation of higher, compared with the low-dose group, the difference was statistically significant (P <0.05). Conclusion: The middle dose of safflower injection for the treatment of chronic spinal cord injury significantly increased the effect of other doses, this dose can be used as safflower injection for chronic spinal cord injury reference dose.