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目的:观察褪黑激素对β-淀粉样多肽25-35片段(Nβ_(25-35)所致神经细胞毒性作用的影响.方法:用相衬倒置显微镜观察原代培养的神经细胞的形态;用噻唑兰(MTT)法、乳酸脱氢酶(LDH)测定及台盼兰染色观察神经细胞的存活情况.结果:Aβ_(25-35)(20μmol/L)可引起培养的神经细胞数量减少,部分突起消失;台盼兰着色细胞数增加;神经细胞增殖能力降低;培养上清液中LDH释放量增加;褪黑激素(Ⅰ,10μmol/L)可抑制Aβ_(25-35)诱导的上述毒性损伤.结论:Aβ_(25-35)对原代培养的皮质-海马神经细胞有直接的细胞毒作用,褪黑激素对Aβ_(25-35)所致神经细胞毒性损伤具有剂量依赖性的防护作用.
Objective: To observe the effect of melatonin on the neurotoxicity of β-amyloid peptide 25-35 (Nβ_ (25-35)) in vitro.Methods: The morphological changes of primary cultured neurons were observed by inverted phase contrast microscopy (MTT), lactate dehydrogenase (LDH) and trypan blue staining were used to observe the survival of nerve cells.Results: Aβ 25-35 (20μmol / L) could reduce the number of cultured neurons, The number of cells in the testis increased, the number of the cells in trypan blue increased, the proliferation of nerve cells decreased, and the release of LDH in the culture supernatant increased. Melatonin (Ⅰ, 10μmol / L) inhibited the above-mentioned toxicity induced by Aβ 25-35 .Conclusion: Aβ_ (25-35) has a direct cytotoxic effect on primary cultured cortical-hippocampal neurons. Melatonin has a dose-dependent protective effect on neurotoxic injury induced by Aβ_ (25-35).