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应用外周微量血培养加细胞松弛素B,观察微核的方法(简称微量CB法),观察~(60)Coγ线低剂量照射诱发的微核和不同年龄组的自发微核,并与常规培养微核法(简称CM法)比较。结果表明外周微量血直接培养,亦获得良好的细胞质分裂阻断微核。两种方法不同年龄组自发微核率,微量CB法的为4.14‰,呈线性正相关。CM法的为6.11‰,随年龄的增长而增多。其低剂量~(60)Coγ线照射诱发的微核,微核率与剂量都有很好的相关性。拟合直线回归方程分别为Y=6.01±0.692D和Y=0.486D-1.968。比较两种方法,CB法更灵敏和准确可靠,可为生物剂量计。
Peripheral blood cultures were incubated with cytochalasin B to observe the micronuclei (micronuclei) method. The micronuclei induced by ~ (60) Coγ-ray irradiation and spontaneous micronuclei in different age groups were observed and compared with routine culture Micronucleus method (referred to as CM method) comparison. The results showed that the peripheral micro-blood culture directly, but also to obtain a good cytoplasmic cleavage of micronuclei. The spontaneous micronucleus rate of the two methods in different age groups, trace CB method was 4.14 ‰, a positive linear correlation. The CM method is 6.11 ‰, which increases with age. The micronucleus and micronucleus rate induced by low dose of ~ (60) Coγ ray irradiation had good correlation with the dosage. The fitting linear regression equations were Y = 6.01 ± 0.692D and Y = 0.486D-1.968, respectively. Comparing the two methods, the CB method is more sensitive and accurate and reliable, and can be a biological dosimeter.