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目的:研究松花粉对衰老小鼠肾脏线粒体DNA(mtDNA)缺失突变的影响。方法:取昆明种衰老小鼠随机分为松花粉处理组和老年对照组,同时随机选取1组为青年对照组。松花粉处理组每天给予750 mg.kg-1的松花粉灌胃;青年对照组与老年对照组以等体积的0.9%氯化钠注射液灌胃。连续60 d后,采用聚合酶链反应(PCR)技术和光密度扫描检测3组mtDNA的缺失突变情况;测定小鼠肾脏组织中SOD活性、MDA含量。结果:老年对照组小鼠肾脏mtDNA含量及mtDNA缺失明显增多(P<0.05);与老年对照组比较,松花粉组能显著减少衰老小鼠肾脏mtDNA的缺失(P<0.05)。松花粉能明显增加衰老小鼠肾脏组织中SOD活性及减少衰老小鼠肾脏MDA含量(P<0.05)。结论:松花粉可以抑制衰老小鼠肾脏mtDNA的缺失突变,提示松花粉能减少mtDNA的氧化损伤,对mtDNA有保护作用,从而从分子水平提供了松花粉延缓衰老的可能机制。
Objective: To study the effect of pine pollen on the deletion of mitochondrial DNA (mtDNA) in the kidney of senile mice. Methods: The aged mice of Kunming were randomly divided into the pine pollen-treated group and the elderly control group. At the same time, 1 group was randomly selected as the young control group. The pine pollen-treated group was given 750 mg · kg-1 of pine pollen orally daily while the young control group and the elderly control group were orally administered with an equal volume of 0.9% sodium chloride injection. After 60 days, the mtDNA deletion mutation was detected by polymerase chain reaction (PCR) and optical density scanning. The activity of SOD and the content of MDA in the kidney of mice were measured. Results: Compared with the control group, the mtDNA content and mtDNA deletion in the kidney of the aged control group were significantly increased (P <0.05). Compared with the old control group, the pine pollen group could significantly reduce the mtDNA deletion in the kidney of the aged mice (P <0.05). Pine pollen can significantly increase the activity of SOD in the kidney of aged mice and decrease the content of MDA in the kidney of aging mice (P <0.05). CONCLUSION: Pine pollen can inhibit the deletion mutation of mtDNA in the kidney of aging mice, suggesting that pine pollen can reduce the oxidative damage of mtDNA and protect mtDNA, which may provide a possible mechanism of pine pollen to delay aging at the molecular level.