,Changes of phenotype and function of human CD4+CD25-T cells induced by transfection of Foxp3

• 出 处: 中国高等学校学术文摘·医学
• 发表日期: 2008年4期
• 关键词: Forkhead transcription factors FOXP3 pro-tein human T-lymphocytes regulatory Ret

The aim of this paper is to explore the effects of transfection of Foxp3 gene on the phenotype and function of naive CD4+T cells. The pMSCV-Foxp3 retroviral vec-tor encoding Foxp3 gene was transduced into the PT67 packaging cell line. Virus-containing supeatant was applied to differentiate CD4+CD25- T cells. The resulting cells were sorted with flow cytometry. The expressions of CD25, CD127, CTLA-4 and the proliferation of trans-fected T cells were examined. The effect of transfected CD4+T cells on the proliferation and cytokine production of CD4+CD25- T cells was examined. Foxp3-gene trans-fected CD4+ T cells could express Foxp3 and transfection of Foxp3 gene up-regulated the expressions of CD25 and CTLA-4, but down-regulated CD127 expression. After transfection, the proliferation of CD4+ T cells was elimi-nated. Transfected T cells inhibited the proliferation of CD4+CD25- T cells. CD4+CD25-T cells acquired a reg-ulatory phenotype and function after it was transduced with the Foxp3 gene. This suggested a key role of Foxp3 in the generation of CD4+CD25+ regulatory T cells.