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目的:获得人源性抗HBsAg抗体Fab片段。方法:用预包被HBsAg的ELISA板,从构建的人全套抗体库中筛选出针对HBsAg的噬菌体抗体。并转入大肠杆菌中表达。破裂细胞后,获得可溶性Fab片段。以羊抗人Fab抗体偶联HiTrap柱,用
Objective: To obtain human anti-HBsAg antibody Fab fragment. Methods: The phage antibody to HBsAg was screened from the constructed human whole antibody library by ELISA plate pre-coated with HBsAg. And transferred into E. coli expression. After the cells are broken, a soluble Fab fragment is obtained. Goat anti-human Fab antibody coupled HiTrap column