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本研究以明亮发光杆菌作为指示生物，将细胞固定化技术、生物传感器技术与发光细菌毒性测试技术有机结合起来，建立一种细菌发光传感器．确定了固定化菌膜的发光强度及稳定时间．以这一检测系统测定了３种金属离子及３种有机化合物的急性毒性（以抑制菌膜发光强度５０％所需的受试物浓度ＥＣ５０值表示），并分析了毒性作用的动力学过程．实验结果表明：在ｐＨ７．０，温度２０℃，３．０％ＮａＣｌ底液条件下，固定化菌膜的发光强度达２５０－３００×１０－７ｍＷ，稳定时间达６０－８０ｍｉｎ；各受试物毒性强弱及ＥＣ５０值（ｍｇ／Ｌ）为：Ｈｇ２＋（０．１５）＞Ｃｕ２＋（１４）＞Ｚｎ２＋（１３０），苯酚（３５）＞乙醛（２１０）＞醋酸乙酯（１２００），与哺乳动物毒性试验的ＬＤ５０值顺序一致，有较高的灵敏度和稳定性；不同毒物之间对细菌发光反应的抑制速率有差异．
In this study, a bright light-emitting bacterium was used as an indicator organism. The cell immobilization technology, the biosensor technology and the luminescent bacteria toxicity testing technology were organically combined to establish a bacterial luminescence sensor. The luminescent intensity and the stabilization time of immobilized bacterial membrane were determined. The acute toxicity of three kinds of metal ions and three kinds of organic compounds was measured by this detection system (the EC50 value of the test substance required to inhibit 50% of the luminescent intensity of the film) and the kinetic process of toxicity was analyzed. The experimental results show that under the conditions of pH 7.0, temperature 20 ℃ and 3.0% NaCl, the luminescent intensity of the immobilized membrane reaches 250-300 × 10-7mW and the stabilization time reaches 60-80min. Toxicity and EC50 values were as follows: Hg2 + (0.15)> Cu2 + (14)> Zn2 + (130), phenol (35)> acetaldehyde (210)> ethyl acetate The LD50 values of animal toxicity test were consistent, with high sensitivity and stability. The rates of inhibition of luminescent reaction to different toxicants varied.