,D609 induces vascular endothelial cells and marrow stromal cells differentiation into neuron-like c

• 作 者: Nan WANG Chun-qing DU Shao-shan WANG Kun XIE Shang-li ZHANG Jun-ying MIAO
• 机 构: Institute of Developmental Biology,School of Life Science,Shandong University,Ji-nan 250100,China
• 出 处: 中国药理学报（英文版）
• 发表日期: 2004年4期
• 关键词: tricyclodecane-9-yl-xanthogenate cell differentiation vascular endothelial cells

AIM: To investigate the effect of tricyclodecane-9-yl-xanthogenate (D609) on cell differentiation in vascular endothelial cells (VECs) and marrow stromal cells (MSCs). METHODS: Morphological changes were observed under phase contrast microscope. Electron microscope and immunostaining were used for VECs identification. The expressions of neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) were examined by immunohistochemistry. RESULTS: After 6 h of induction with D609, some VECs showed morphological changes characteristic of neurones. 9 h later, more VECs became neuron-like cells. About 30.8 % of VECs displayed positive NSE (P<0.01), while the expression of GFAP was negative. When MSCs were exposed to D609, the cells displayed neuronal morphologies, such as pyramidal cell bodies and processes formed extensive networks at 3 h. 6 h later, almost all of the cells exhibited a typical neuronal appearance, and 85.6 % of MSCs displayed intensive positive NSE, but GFAP did not express. CONCLUSION: D609 induces VECs and MSCs differentiation into neuron-like cells.