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Ionic liquids are a type of salts that are liquid at low temperature (<100 ℃). Because of their some special properties, they have been widely used as new 揼reen solvents?for many chemical reactions and liquid-liquid ex-traction in the past several years. In this paper, a new method for the separation of nucleotides is developed and the essential feature of the method is that 1-alkyl-3-methylimidazolium salts are used as mobile phase additives, result-ing in a baseline separation of nucleotides without need of gradient elution and need of organic solvent addition as currently used in RP-HPLC. This study shows the potential application of ionic liquids as mobile phase additives in reversed-phase liquid chromatography.
Ionic liquids are a type of salts that are liquid at low temperatures (<100 ° C.). Because of some special properties, they have been widely used as new solvents. For many chemical reactions and liquid-liquid ex-traction in the past this years, in this paper, a new method for the separation of nucleotides is developed and the essential feature of the method is that 1-alkyl-3-methylimidazolium salts are used as mobile phase additives, result-ing in a baseline separation of nucleotides without need of gradient elution and need of organic solvent addition as currently used in RP-HPLC. This study shows the potential application of ionic liquids as mobile phase additives in reversed-phase liquid chromatography.