论文部分内容阅读
本文调查了１５株粘鞭菌属Ｇｌｉｏｍａｓｔｉｘｌｕｚｕｌａｅ菌种产生环孢菌素Ｃ的能力。分离自中国１１省１５个不同地区的菌种在葡萄糖－麦芽汁－蛋白胨液体培养基中于２４～２６℃发酵１０ｄ。代谢产物经ＨＰＬＣ分析，其中１１株菌株除能产生环孢菌素Ｃ外不产生任何已知的环孢菌素，另４株菌虽然不产生已知的环孢菌素，但全部都产生一未知的环孢菌素。对产生环孢菌素Ｃ的两株具有不同液体培养性状的菌株用Ｌ－丙氨酸、Ｄ－丙氨酸、Ｌ－缬氨酸、ＤＬ－α－氨基丁酸、ＤＬ－原缬氨酸和Ｌ－苏氨酸进行氨基酸前体掺入实验，发现所有氨基酸前体均只能调节菌株合成环孢菌素Ｃ的能力，而不能使其合成相应的其它环孢菌素，其中Ｌ－丙氨酸和Ｄ－丙氨酸具有相近但比其他氨基酸更强的提高菌株合成环孢菌素Ｃ的能力，提示该类菌的合成机制与已知环孢菌素Ａ产生菌的合成机制明显不同。
In this paper, the ability of 15 strains of Clostridium Gliomastixluzulae to produce cyclosporin C was investigated. The strains isolated from 15 different areas in 11 provinces of China were fermented in glucose-wort-peptone liquid medium at 24 ~ 26 ℃ for 10 days. The metabolites were analyzed by HPLC, of which 11 strains did not produce any known cyclosporin except cyclosporin C and the other 4 strains, although not producing the known cyclosporins, all produced one Unknown cyclosporin. Two isolates producing cyclosporin C with different liquid culture traits were treated with L-alanine, D-alanine, L-valine, DL-α-aminobutyric acid, DL-norvaline And L-threonine amino acid precursor incorporation experiments found that all amino acid precursors can only regulate the ability of strains to synthesize cyclosporin C, but can not make the corresponding synthesis of other cyclosporins, which L- The similar ability of the amino acid and D-alanine to enhance the synthesis of cyclosporine C was stronger than that of other amino acids, suggesting that the mechanism of synthesis of this bacterium is obviously different from that of known cyclosporin A producing bacteria .