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用高效液相色谱外标法测定了木奈果中的可溶性糖和山梨醇 ,采用 μ Spherogelcarbohydrate柱和示差折光检测器 ,以水为流动相 ,柱温 85℃。相关系数在 0 970 9以上 ,加标回收率为 75 0 0 %～ 88 37%。测定结果表明 ,可溶性糖在果肉中的累积依果肉不同的生长发育阶段有特征性的变化。
Soluble sugars and sorbitol were determined by RP-HPLC with μ Spherogelcarbohydrate column and differential refractive index detector. The mobile phase consisted of water at 85 ℃. The correlation coefficient was over 0 970 9, and the recoveries were 75 0 0% ~ 88 37%. The results showed that the accumulation of soluble sugars in the flesh varied markedly in different stages of growth and development of flesh.