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采用微波辅助丙酮/硫酸铵两相盐析体系萃取万寿菊花中的酚类物质及叶黄素。通过单因素实验,考察了微波作用时间、微波功率、料液比对目标产物在两相间分配行为的影响,确定最优萃取条件,并采用响应曲面法优化微波辅助盐析萃取万寿菊花中多酚的提取工艺。结果表明,多酚、类黄酮及叶黄素富集于上相丙酮相,得率分别为85.76、6.70、0.85 mg/g;回收率分别为96.64%、98.15%、95.98%。响应曲面法优化微波辅助盐析萃取万寿菊花中多酚的最优提取工艺为:微波功率500 W,微波作用时间95 s,料液比(g/m L)为1∶45,粉碎目数160目,在该条件下,多酚得率为86.24 mg/g,并对提取物抗氧化活性作了初步测试,结果表明,提取物对DPPH·及ABTS·+有明显的清除效果,其抗氧化活性优于两相盐析提取物。
Extraction of phenols and lutein from marigold flowers by microwave assisted acetone / ammonium sulfate two - phase salting - out system. Through the single factor experiment, the effects of microwave action time, microwave power and solid-to-liquid ratio on the distribution of the target product between the two phases were investigated to determine the optimal extraction conditions. The response surface methodology was used to optimize the extraction of polyphenols The extraction process. The results showed that the polyphenols, flavonoids and lutein were enriched in the phase of the upper phase acetone, the yield was 85.76,6.70,0.85 mg / g, the recovery rates were 96.64%, 98.15%, 95.98% respectively. Response surface method optimization of microwave-assisted salting extraction of polyphenol in marigold flower optimum extraction process: microwave power 500 W, microwave action time 95 s, the material liquid ratio (g / m L) of 1:45, the number of crushed 160 Under these conditions, the yield of polyphenols was 86.24 mg / g, and the antioxidant activity of the extracts was preliminary tested. The results showed that the extract had obvious scavenging effects on DPPH · and ABTS · +, and its antioxidant activity Activity better than two-phase salting extract.