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〔目的〕了解EHECO157∶H7在云南战区部队腹泻患者和战区猪、牛和污水中的存在情况。〔方法〕在SMAC琼脂平板上分离 ,快速MUG试验初筛 ,用VITEK3 2 全自动微生物生化分析仪鉴定 ,复合PCR扩增法检测毒素基因。〔结果〕从人、猪、牛中分别检出EHECO157∶H7和EHECO157:H-,其中O157:H7检出三个毒力基因。〔结论〕云南战区至少存在两种不同类型的EHECO157,要警惕和预防此菌的感染。
[Purpose] To understand the presence of EHECO157: H7 in diarrhea units and war zones pigs, cattle and sewage in Yunnan theater units. [Method] On SMAC agar plates, rapid MUG test was carried out, and VITEK3 2 automatic biochemical analyzer was used to identify the toxin. The toxin gene was detected by multiplex PCR amplification. [Results] EHECO157: H7 and EHECO157: H- were detected in human, porcine and bovine, respectively, and three virulence genes were detected in O157: H7. [Conclusions] There are at least two different types of EHECO157 in the theater of war in Yunnan Province, so we should guard against and prevent the infection of this bacterium.