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采用纤维素膜技术研究了大蒜、洋葱、芹菜、丁香、花椒、大黄等 2 4种植物离体组织及其提取物(其中 8种中草药为水煎剂 ,16种其它植物为水浸剂 )对马铃薯晚疫病菌 ( Phytophthora infestans)静止胞萌发、附着胞形成及侵入丝形成的直接影响和提取物诱导块茎对晚疫病病菌侵入机构形成的影响 ,并在此基础上进一步就提取物免疫处理种薯、诱导成株期抗病性、增加块茎产量的影响做了初步探讨。在洋葱、葱、大蒜等 12种植物组织表面 ,静止胞萌发率明显低于对照 (擦镜纸 ) ,而且在 6种植物组织表面未见附着胞形成 ,10种植物组织表面未见侵入丝形成 ;在大蒜、韭菜等 13种植物组织提取液中 ,静止胞萌发率明显低于对照 (擦镜纸 ) ,其中 6种植物组织提取液中附着胞未见形成 ,7种植物组织提取液中侵入丝未见形成。经 8种植物组织提取物诱导处理的块茎切片 ,不仅保鲜期长 ,而且表现出较强的诱导抗性 ;静止胞萌发率、附着胞形成率及侵入丝形成率均明显低于对照 ;经 8种植物组织提取物诱导处理的种薯在出芽、早期生长、抗晚疫病及产量等各方面均优于对照 ,病害保护率达54%、增产率达 31%。
Cellulose membrane technology was used to study the isolated tissues and extracts of 24 species of plants such as garlic, onion, celery, clove, pricklyash and rhubarb (8 kinds of Chinese herbal medicines were water-decoction and 16 kinds of other plants were aqueous) The direct effect of the formation of adherent cells and invasion of Phytophthora infestans, and the effect of tuber inducing tubers on the invasion mechanism of Phytophthora infestans, and on this basis, , Inducing adult plant disease resistance, increasing the impact of tuber yield made a preliminary study. On the surface of 12 plant tissues such as onion, onion and garlic, the germination rate of quiescent cells was significantly lower than that of the control (lens cleaning paper), and no adherent cells were formed on the surface of the 6 plant tissues, and no invasion silk formed on the surface of 10 plant tissues The germination rate of quiescent cells in garlic, leeks and other 13 kinds of plant tissue extracts was significantly lower than that of the control (lens cleaning paper), of which 6 kinds of plant tissue extracts did not form adherent cells, and 7 kinds of plant tissue extracts invaded No formation of silk The tuber slices induced by 8 kinds of plant tissue extracts not only had long shelf life, but also showed strong induction resistance. The germination rate of stationary cells, the rate of adherent cells and the rate of invading silk were significantly lower than that of the control. Seed treatment induced by plant tissue extracts was better than control in the aspects of budding, early growth, resistance to late blight and yield, with the protection rate of 54% and the yield increasing rate of 31%.