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报道用高效毛细管电泳技术快速测定内江猪血清头孢唑啉钠（ｃｅｆａｚｏｌｉｎｓｏｄｉｕｍ，ＣＥＺ）的方法。血清用乙腈去除蛋白质，经离心后上清直接进样，毛细管内径７５μｍ，有效长度４０ｃｍ，在５０ｍｍｏｌ／Ｌ碳酸钠／碳酸氢钠缓冲液（ｐＨ１０）中，电压２０ｋＶ分离５分钟，２５４ｎｍ紫外检测。此方法操作简单，测定迅速，线性范围为５～３２０μｇ／ｍｌ，日内相对标准偏差为１．７％，日间相对标准偏差为８．０％，平均加样回收率为１０７．９％，最低检测限为１．５μｇ／ｍｌ，定量灵敏度为３．６μｇ／ｍｌ。按１６ｍｇＣＥＺ／ｋｇ对６头成年内江猪作静脉注射，测定结果经药动学软件分析得到药代动力学参数，结果表明内江猪对ＣＥＺ的代谢为二室开放模型，清除率为８．４３ｍｌ／（ｋｇ·ｍｉｎ）。
A rapid method for the determination of cefazolium sodium (CEZ) in Neijiang pigs by high performance capillary electrophoresis was reported. The serum was removed by acetonitrile, and the supernatant was directly injected after centrifugation. The inner diameter of the capillary was 75 μm and the effective length was 40 cm. The separation was performed at 50 kV for 20 min in 50 mmol / L sodium carbonate / sodium bicarbonate buffer solution (pH 10). The method is simple, rapid determination, the linear range of 5 ~ 320μg / ml, the relative standard deviation of 1.7%, day relative standard deviation of 8.0%, the average recovery was 107.9%, the lowest The detection limit was 1.5μg / ml and the quantitative sensitivity was 3.6μg / ml. Six adult Neijiang pigs were injected intravenously at 16 mg CEZ / kg. The pharmacokinetic parameters of the Neijiang pig were determined by pharmacokinetic analysis. The results showed that Neijiang pig’s metabolism of CEZ was a two-compartment open model with a clearance rate of 8.43 ml / (Kg · min).