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目的研究白藜芦醇(resveratrol,RES)对呼吸道合胞病毒(respiratory syncytial virus,RSV)感染小鼠肺部病毒滴度及其所致气道炎症的影响。方法 6～8周龄雌性BALB/c小鼠,环磷酰胺100 mg/kg腹腔注射后,随机分为对照组、RSV感染组、白藜芦醇腹腔注射组、白藜芦醇雾化组,每组8只。环磷酰胺处理5 d后,给予RSV滴鼻建立RSV感染模型,并在RSV感染1 h后给予白藜芦醇30 mg/(kg.d)腹腔注射和雾化吸入。5 d后检测小鼠清醒状态下气道阻力,处死小鼠研究肺部RSV病毒滴度、肺泡灌洗液(BALF)中细胞计数、肺部病理组织炎症及评分。结果白藜芦醇不同方式给药,干预组肺部RSV病毒滴度均显著低于RSV感染组(P<0.01),BALF中细胞总数白藜芦醇干预组明显低于RSV感染组(P<0.05),而白藜芦醇干预组与对照组相比差异无显著性;肺组织炎症病理评分:白藜芦醇干预组明显低于RSV感染组(P<0.01),白藜芦醇干预组清醒状态小鼠气道高反应(吸入乙酰甲胆碱浓度12.5～50.0 mg/ml时)较单纯RSV感染组明显减轻(P<0.05)。结论白藜芦醇不同方式给药均能够减轻RSV诱发的肺部炎症及气道高反应,其机制可能是通过抑制RSV感染后肺部病毒滴度,减少RSV在体内的复制。
Objective To investigate the effects of resveratrol (RES) on pulmonary virus titer and airway inflammation in mice infected with respiratory syncytial virus (RSV). Methods BALB / c mice of 6-8 weeks old were injected intraperitoneally with 100 mg / kg cyclophosphamide and randomly divided into control group, RSV infection group, resveratrol intraperitoneal injection group, resveratrol atomization group, Each group of 8. Five days after the treatment with cyclophosphamide, RSV was given intranasally to establish RSV infection model. Resveratrol 30 mg / (kg · d) was given intraperitoneally and nebulized after 1 h of RSV infection. After 5 days, the airway resistance of the mice in awake state was detected. The mice were sacrificed to study the pulmonary RSV virus titer, cell count in BALF, inflammation of lung pathology tissue and score. Results Resveratrol administered in different ways showed that the RSV viral titer in the lungs of the intervention group was significantly lower than that of the RSV group (P <0.01), and the total number of cells in the BALF was significantly lower than that of the RSV group (P < 0.05). There was no significant difference between resveratrol intervention group and control group. Inflammatory pathological score of resveratrol group was significantly lower than that of RSV infection group (P <0.01), resveratrol intervention group The airway hyperresponsiveness in awake mice (12.5 ~ 50.0 mg / ml inhalation of methacholine) was significantly lower than that of RSV alone (P <0.05). Conclusions Resveratrol can reduce lung inflammation and airway hyperresponsiveness induced by RSV in different ways. The mechanism may be that it inhibits the virus titer in the lung after RSV infection and reduces the in vivo replication of RSV.