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应用Biolog Eco微平板培养法研究了中国科学院海伦农业生态试验站20余年连续不同施肥处理(对照、氮磷钾、氮磷钾+秸秆、氮磷钾+猪粪1、氮磷钾+猪粪2和氮磷钾+猪粪3)下土壤微生物代谢功能多样性的变化。结果表明:长期施肥条件下,单施化肥和有机肥与化肥配施对土壤细菌、真菌数量影响不明显,但高量有机肥与化肥配施可以显著增加放线菌数量。Biolog结果表明,施用有机肥的平均颜色变化率高于单施化肥和对照。Shannon指数、Simpson指数和McIntosh指数均是有机肥和化肥配施高于化肥单施与对照。主成分分析结果表明,对照、NPK与其他几种处理有较明显的距离,说明有机肥的连年施用导致了土壤微生物代谢功能多样性的变化。
Biolog Eco microtiter plate culture method was used to study the effects of continuous fertilization (control, NPK, NPK + straw, NPK + pig manure 1, NPK + pig manure 2 for more than 20 years at Helen Agricultural Ecology Experimental Station, Chinese Academy of Sciences And phosphorus and phosphorus + pig manure 3) microbial metabolic function diversity changes. The results showed that under the condition of long-term fertilization, the amount of actinomyces was significantly increased with the application of chemical fertilizers and organic manure and chemical fertilizers on soil bacteria and fungi. The results of Biolog showed that the average color change rate of organic fertilizer was higher than that of chemical fertilizer and control. Shannon index, Simpson index and McIntosh index were higher than those of chemical fertilizer and organic fertilizer. The results of principal component analysis showed that NPK had obvious distance from other treatments, which indicated that the successive application of organic fertilizer resulted in the change of metabolic function and diversity of soil microorganisms.