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采用高效液相色谱法同时测定喘静片中的 3种主要成分 :茶碱、异戊巴比妥和盐酸甲基麻黄碱。以ODS作固定相 ,通过均匀设计方法选择的最佳流动相为V (0 0 1 5mol/L磷酸二氢钠缓冲液 ,含体积分数为 0 3%的三乙胺 ,pH 4 9)∶V(甲醇 ) =35∶65,检测波长为 2 1 5nm ,用程序调节检测器的灵敏度 ,以咖啡因作内标物。茶碱、异戊巴比妥、盐酸甲基麻黄碱的回收率分别为 99 7%～ 1 0 2 6%,98 5%～ 1 0 0 2 %,98 0 %～ 1 0 2 7%,日间RSD分别为 0 2 3%～ 1 2 %,0 35%～ 2 5%,0 33%～ 1 6%。该方法简便、快速、准确。
Simultaneous determination of three main components of asthma tablets by high performance liquid chromatography: theophylline, amobarbital and methyl ephedrine hydrochloride. ODS as the stationary phase, the optimal mobile phase selected by the uniform design method is V (0 0 1 5mol / L sodium dihydrogen phosphate buffer, with a volume fraction of 0.3% triethylamine, pH 4 9): V (Methanol) = 35:65, detection wavelength of 2 1 5nm, with the program to adjust the sensitivity of the detector to caffeine as an internal standard. The recoveries of theophylline, amobarbital and methyl ephedrine hydrochloride were 99.7% -102.6%, 98.5% -100.2% and 98.0% -102.7%, respectively The RSDs were between 0 2 3% and 1 2%, 0 35% and 25%, 0 33% and 1 6%, respectively. The method is simple, fast and accurate.