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目的建立盆腔积液结核分枝杆菌特异性IFN-γ酶联免疫斑点(PEMC Elispot)检测技术,并与相应的外周血检测结果比较。方法以重组结核菌早期分泌抗原6k Da蛋白(ESAT-6)、ESAT-6重组多肽抗原组合(P8.10)及多肽抗原组合(P8.10+Pool B)为抗原,建立盆腔积液结核分枝杆菌特异性IFN-γElispot检测技术(PEMC Elispot)。对46例确诊为结核性盆腔炎病人的盆腔积液进行结核菌特异性IFN-γ水平检测;同时用外周血结核菌特异性IFN-γ水平检测技术对病人相应的外周血进行检测,并用PEMC Elispot检测了30例非结核性盆腔炎病人积液标本。结果 PEMC Elispot技术对46例确诊为结核性盆腔炎病人盆腔积液检测,阳性率为97.8%;PBMC ELlispot检测病人外周血,阳性率为87.0%,经用四格表确切概率法统计,差异有统计学意义(χ2=6.815,P=0.009);PEMC Elispot检测非结核性盆腔炎病人盆腔积液的阳性率为10.0%,显著低于结核性盆腔炎病人盆腔积液阳性率(χ2=60.19,P<0.0001)。结论盆腔积液结核菌特异性IFN-γElispot检测技术与外周血结核杆菌特异性IFN-γElispot检测技术一样在结核性盆腔炎诊断应用中有着较好的敏感性和特异性,可以作为结核性盆腔炎诊断及其鉴别诊断的方法。
Objective To establish the detection method of Mycobacterium tuberculosis specific IFN-γ ELISA for pelvic fluid effusion and compare with the corresponding peripheral blood test results. Methods The tuberculosis secretions and tuberculosis were established with 6k Da protein (ESAT-6), ESAT-6 recombinant peptide antigen (P8.10) and P8.10 + Pool B antigen Mycobacterial-specific IFN-γ Elisapot assay (PEMC Elispot). Mycobacterium tuberculosis specific IFN-γ was detected in 46 cases of pelvic fluid diagnosed as tuberculous pelvic inflammatory disease. At the same time, the corresponding peripheral blood of patients with tuberculous meningitis specific IFN-γ was detected by PEMC Elispot tested 30 patients with non-tuberculous pelvic inflammatory disease effusion specimens. Results PEMC Elispot technique was used to detect pelvic fluid in 46 patients diagnosed as tuberculous pelvic inflammatory disease. The positive rate was 97.8%. PBMC ELISpot was used to detect peripheral blood of patients with a positive rate of 87.0% (Χ2 = 6.815, P = 0.009). The positive rate of pelvic fluid in patients with non-tuberculous pelvic inflammatory disease detected by PEMC Elispot was 10.0%, which was significantly lower than that of pelvic fluid in tuberculous pelvic inflammatory disease (χ2 = 60.19, P <0.0001). Conclusions The tuberculous pelvic inflammatory disease-specific IFN-γElispot detection technique has the same sensitivity and specificity as the Mycobacterium tuberculosis-specific IFN-γElispot detection technique in the diagnosis and diagnosis of tuberculous pelvic inflammatory disease. It can be used as tuberculous pelvic inflammatory disease Diagnosis and differential diagnosis of the method.