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近年来各种实验技术有了很大发展,传统的体内动物实验方法虽有操作简单、接近实际的优点,但只能反映肿瘤大体的生长状态,用以比较宏观的变化;离体细胞存活曲线方法虽然定量性好,但单层贴瓶的生长状态与实体瘤相差甚远。自Barendsen首次直接原代培养动物肿瘤细胞观察其存活状况成功以后,整体实验离体分析(in vivo-in vitro excision assays)方法被广泛采用。用此法绘制的存活曲
In recent years, various experimental techniques have developed greatly. Although traditional in vivo animal experimental methods have the advantages of simple operation and close to actuality, they can only reflect the general growth state of tumors, and can be used to compare macroscopic changes; ex vivo cell survival curves. Although the method is of good quantification, the growth status of single-layer paste bottles is far from that of solid tumors. Since Barendsen’s first direct primary culture of animal tumor cells to observe its survival was successful, in vivo-in vitro excision assays were widely used. The survival curve drawn by this method