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目的探讨建立稳定糖尿病合并梅毒兔模型的方法,观察其14周病程发展过程,并在病程结束后,观察实验兔心脏、肺、肝脏、肾脏、睾丸组织病理损伤情况并初步探讨原因。方法四氧嘧啶诱导法构建稳定的单独糖尿病兔模型(DM组);睾丸接种梅毒螺旋体感染梅毒构建稳定梅毒兔模型(Tp组);先四氧嘧啶诱导稳定糖尿病,继以睾丸接种梅毒螺旋体致使其感染梅毒构建稳定糖尿病合并梅毒兔模型(H组),另设健康对照组(C组),动态观察各组兔临床表现,如食量、饮水量、体重、尿量、毛色、精神状态等改变情况,并在实验的14周解剖兔,对其心脏、肺、肝脏、肾脏、睾丸组织做病理切片、HE染色,观测各组织病理损伤情况及初步探讨其原因。结果成功构建了单独糖尿病组、单独梅毒组及糖尿病合并梅毒组兔模型。实验过程中,单独梅毒组、单独糖尿病组、糖尿病合并梅毒组兔其饮食量、饮水量、体重、尿量、毛色、精神状态等出现了不同程度的变化。14周实验结束时,与健康对照组比较,单独梅毒组、单独糖尿病组、糖尿病合并梅毒组兔各实验组器官表现出一定的病理改变,其中心脏组织出现水肿、炎症等病理改变;肺组织出现炎性细胞浸润的改变;肝脏组织出现细胞肿胀、炎症甚至点状坏死等改变;肾脏组织出现水肿、血管扩张、炎症甚至蛋白管型及钙盐沉积等明显病理变化;睾丸组织出现明显间质细胞增生、多种炎性细胞浸润及结节坏死现象;与单独疾病相比,H组各组织的病理变化更显著。结论糖尿病合并梅毒兔模型在心脏、肺、肝脏、肾脏、睾丸组织病理方面,能引起比单独疾病组更严重的组织病理损伤,其可能机制与合并感染加速了机体的氧化应激导致出现更严重的炎症反应有关。
OBJECTIVE: To establish a stable diabetic model of syphilis in rabbits and to observe its course of development in 14 weeks. After the course of the disease, the histopathological damage in the heart, lung, liver, kidney and testis of experimental rabbits was observed and the reasons were discussed. Methods Toxoplasma gondii induced diabetes mellitus (DM). The testis was infected with Treponema pallidum and infected with syphilis to establish a stable syphilis model (Tp group). Alloxan induced stable diabetes, followed by testicular inoculation with Treponema pallidum Infection with syphilis to construct a stable diabetic model with syphilis (group H), another healthy control group (group C), the dynamic observation of the clinical manifestations of rabbits, such as food intake, water intake, weight, urine output, coat color, mental status and other changes Rabbits were dissected 14 weeks after the experiment, pathological sections of heart, lung, liver, kidney and testis were made and stained with hematoxylin and eosin (HE). The histopathological damage of each group was observed and the reasons were preliminarily discussed. Results A single diabetic group, a single syphilis group and a diabetic syphilis group were successfully constructed. During the experiment, the diet, water intake, body weight, urine output, coat color and mental status of syphilis alone, diabetes alone, and diabetes mellitus with syphilis changed in different degrees. At the end of the 14-week experiment, compared with the healthy control group, the experimental organs in the syphilis alone group, the diabetes alone group and the diabetic syphilis group showed some pathological changes, including edema and inflammation in the heart tissue, and the appearance of the lung tissue Inflammatory cells infiltration; liver tissue swelling, inflammation or even necrosis and other changes; renal tissue edema, vasodilation, inflammation and even protein tube and calcium deposition and other significant pathological changes; testicular tissue was significantly stromal cells Hyperplasia, a variety of inflammatory cell infiltration and nodular necrosis; Compared with the disease alone, the pathological changes in each group H more significant. Conclusions The syphilis rabbit model with diabetes mellitus can cause more serious pathological damage in the heart, lung, liver, kidney and testis than the single disease group. The possible mechanism and co-infection may exacerbate the oxidative stress in the body. Inflammatory reaction.