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目的研究LNCap和DU145细胞在体外培养时相关细胞特性及差异。方法常规培养LNCap和DU145细胞株。设6组:无血清空培养液组(A组)、有血精空培养液组(B组)、无血清培养LNCap组(C)组、有血清培养LNCap组(D组)、无血清培养DU145组(E组)和有血清培养DU145组(F组)。6组同时培养48h后,取各自培养液上清,经相关试剂盒ELISA分别检测睾酮(T)、雌二醇(E2)、孕酮(P)、血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)、tPSA和fPSA浓度。结果T在A组未检出,B组因含血清而有少量T,在C、D和F组中T显示低浓度,但在E组却成倍升高。C、D、E和F组E2水平都高于对照组A、B组(P<0.05)。P在6组中差别都不大。VEGF在C、D、E和F组都有极其异常高表达(P<0.01),C、D组比对照组约高100倍,而E、F组比对照组则远高出1000倍。bFGF在C、D组与对照组差别不大,但在E、F组却显著升高(P<0.01)。E、F组中fPSA含量大于C、D组,在D、E和F组fPSA/tPSA均升高达44%。结论研究LNCap和DU145体外培养相关特性,可为我们进一步研究前列腺癌机制提供依据。
Objective To study the cell characteristics and differences of LNCap and DU145 cells cultured in vitro. Methods The LNCap and DU145 cell lines were routinely cultured. There were 6 groups: serum-free empty medium group (group A), blood empty medium group (group B), serum-free culture LNCap group (group C), serum-cultured LNCap group DU145 group (group E) and serum-cultured DU145 group (group F). Six groups were cultured at the same time for 48h, and the supernatant of each culture medium was taken. The levels of testosterone (T), estradiol (E2), progesterone (P), vascular endothelial growth factor (VEGF) Fibroblast growth factor (bFGF), tPSA and fPSA concentrations. Results T was not detected in group A, T in serum in group B was low, T was low in groups C, D and F but doubled in group E. E2 levels in groups C, D, E and F were higher than those in groups A and B (P <0.05). P is not very different in 6 groups. The levels of VEGF in group C, D, E and F were extremely abnormally high (P <0.01), while those in group C and D were about 100 times higher than those in control group, while those in group E and F were 1000 times higher than those in control group. bFGF in C, D group and the control group difference is not significant, but in E, F group was significantly higher (P <0.01). The levels of fPSA in groups E and F were higher than those in groups C and D, and the fPSA / tPSA in groups D, E and F increased by 44%. Conclusion The study of the characteristics of LNCap and DU145 in vitro culture can provide the basis for our further study on the mechanism of prostate cancer.