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利用已建成的人胃癌MGC80—3细胞裸鼠移植瘤为细胞来源,采用机械消化为辅、胶原酶等消化为主,经过改装的三级过滤系统分离活的单细胞,经外科手术,脾被膜下注入1.25×10~6个MGCS0-3细胞。研究表明,移植前施用含0.5％CCl_4橄榄油,使MGC80-3细胞在肝脏形成转移灶数目增多。光镜下,CCl_4中毒后第2天、第10天裸鼠肝脏显示不同程度肝细胞脂肪变性、坏死、纤维化、肝细胞再生和小胆管增生现象。电镜下,CCl_4中毒后肝细胞显示SER,RER不同程度的扩张,并伴有RER脱颗粒,胞质内脂滴和脂褐素增多,肝窦内皮受损收缩,有纤维素样物质沉着,提示CCl_4中毒后肝细胞坏死、内皮损伤、纤粘蛋白沉着是造成MGC80-3细胞肝脏转移灶增多的原因之一。总之利用自系移植成功的裸鼠人胃癌细胞移植瘤为细胞来源,三级细胞过滤系统分离实体瘤组织而获得增殖能力强的单个瘤细胞,借用CCl_4对肝脏损伤,脾下移植亲宿主性胃癌单个细胞,容易获得人胃癌细胞在肝脏多个转移灶。这个方法能为预防和研究肿瘤细胞转移提供可靠而有价值的实验研究模型。
The established human gastric cancer MGC80-3 cell xenografts in nude mice as the source of cells, supplemented by mechanical digestion, collagenase digestion, modified three-stage filtration system to separate living single cells, after surgery, spleen capsule Under the injection of 1.25 × 10 ~ 6 MGCS0-3 cells. Studies have shown that the application of 0.5% CCl_4 olive oil before transplantation, MGC80-3 cells in the liver to form a larger number of metastases. Under light microscope, the liver of nude mice on day 2 and day 10 after CCl_4 poisoning showed varying degrees of hepatic steatosis, necrosis, fibrosis, hepatocyte regeneration and small bile duct hyperplasia. Under electron microscope, the liver cells of CCl_4 poisoning showed different degrees of SER and RER dilatation accompanied with degranulation of RER, increased intracellular lipid droplets and lipofuscin, damaged and constricted hepatic sinusoid endothelial cells, and the presence of cellulosic material CCl_4 poisoning after liver cell necrosis, endothelial injury, fibronectin is one of the causes of increased liver metastases MGC80-3 cells. In short, the use of self-transplantation of human gastric cancer xenografts in nude mice as the source of cells, three-cell filtration system to separate solid tumor tissue to obtain a single cell proliferation ability, borrow CCl 4 on liver injury, Single cells, easy access to human gastric cancer cells in the liver multiple metastases. This method can provide a reliable and valuable experimental research model for the prevention and study of tumor cell metastasis.