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Objective:The aim of this study was to investigate the sensitivity of chemotherapeutic agents 5-FU,cisplatin(DDP) or TAXOL on colon cancer cell line SW480 with different methods,to find out the best examine time period for further study of chemotherapeutic sensitivities.Methods:The SW480 cell was treated with 5-FU(200μg/mL),DDP(150μg/mL) or TAXOL(50μg/mL) respectively for 4h,8h or 12h.MTT assay was used to examine the cell survival rate,Annexin V/PI assay was used to analysis the apoptosis rate,Western Blot assay was applied to examine the expression of apoptotic protein.Results:(1) Results of MTT assay showed that the survival rate of SW480 cells at 4h,8h or 12h was:5-FU(200μg/mL)96.0%±8.2%,85.4%±7.8%,74.4% ±10.2%(P<0.05);DDP(150μg/mL) 99.0%±6.4%,88.7%±4.7%(P<0.05),46.9%±2.6%(P<0.01);TAXOL(50μg/mL) 51.5%±4.2%(P<0.01),31.9%±3.1%,17.6%±2.3%,or blank group 97.2%±5.8%,98.7%±7.2%,97.5%±7.5% respectively.(2) The apoptosis rate of cancer cells at 4h,8h or 12h was:control group:3.4%±0.2%,6.2%±0.4%,7.0%±0.5%;5-FU(200μg/mL) 4.0%±0.3%,4.8%±0.4%,7.7%±0.7%;DDP(150μg/mL) 8.5%±0.9%,18.6%±1.6%(P<0.05),67.0%±6.2%(P<0.01);or TAXOL(50μg/mL) 32.0%±5.2%(P<0.01),76.6%±8.5%,94.0% ±8.2%,respectively.(3) Western Blot assay showed that the expression of apoptosis associated protein.PARP,X-linked inhibitor of apoptasis(XIAP),Caspase-9 and Bcl-xL were changed.Conclusion:The sensitivity of chemotherapy could be assessed by MTT assay,Annexin V/PI assay and Western Blot.The best examine time of the three chemotherapuc agents was 5-FU(200μg/mL):>12h,DDP(150μg/mL):8-12h,or TAXOL(50μg/mL):<4h.
Objective: The aim of this study was to investigate the sensitivity of chemotherapeutic agents 5-FU, cisplatin (DDP) or TAXOL on colon cancer cell line SW480 with different methods, to find out the best examine time period for further study of chemotherapeutic sensitivities. The SW480 cells were treated with 5-FU (200μg / mL), DDP (150μg / mL) or TAXOL (50μg / mL) respectively for 4h, 8h or 12h. MTT assay was used to examine the cell survival rate, Annexin V / PI assay was used to analyze the apoptosis rate, Western Blot was applied to examine the expression of apoptotic protein. Results: (1) Results of MTT assay showed that the survival rate of SW480 cells at 4h, 8h or 12h was: (P <0.05); DDP (150μg / mL) 99.0% ± 6.4%, 88.7% ± 4.7% (P (P <0.05), 46.9% ± 2.6% (P <0.01); TAXOL (51μg / mL) 51.5% ± 4.2% (P <0.01), 31.9% ± 3.1%, 17.6% ± 2.3% 5.8%, 98.7% ± 7.2%, 97.5% ± 7.5% respectively. (2) The apoptosis rate of cancer cells at 4h, 8h or 12h was: control gr oup: 3.4% ± 0.2%, 6.2% ± 0.4%, 7.0% ± 0.5%; DDP 150μg / mL 4.0% ± 0.3%, 4.8% ± 0.4% (P <0.01), and the difference was statistically significant (P <0.01). The percentages of patients receiving TAXOL (50μg / mL) were 8.5% ± 0.9%, 18.6% ± 1.6%, 67.0% ± 6.2% (3) Western Blot assay showed that the expression of apoptosis associated protein. PARP, X-linked inhibitor of apoptotic (XIAP), Caspase-9 and Bcl-xL were changed.Conclusion : The sensitivity of the chemotherapy could be assessed by MTT assay, Annexin V / PI assay and Western Blot.The best examine time of the three chemotherapuc agents was 5-FU (200 μg / mL):> 12h, DDP (150 μg / mL) 8-12h, or TAXOL (50μg / mL): <4h.