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应用与单纯疱疹病毒Ⅰ型（ＨＳＶⅠ）ＵＬ３０基因片段杂交的寡核苷酸探针，对４例未转移浸润癌、４例转移浸润癌及其转移淋巴结组织进行原位杂交研究。结果发现：（１）有４例未转移浸润癌、３例转移浸润癌及其转移淋巴结组织杂交分别阳性，但阳性率无显著性差异；（２）ＵＬ３０基因片段主要位于肿瘤上皮细胞胞浆；（３）ＵＬ３０基因片段在肿瘤浸润和转移的过程持续存留在肿瘤上皮细胞中；（４）存在ＨＳＶⅠ基因片段的组织均无ＨＳＶⅠ增殖感染的形态学特征。提示ＨＳＶⅠ可能以特殊感染方式在口腔鳞状细胞癌的发生发展中起作用。
In situ hybridization was performed on 4 cases of metastatic carcinoma and 4 cases of metastatic lymph node metastasis using oligonucleotide probe hybridizing with HSV Ⅰ type Ⅰ (HSV Ⅰ) UL30 gene fragment. The results showed that: (1) 4 cases of non-metastatic carcinoma, 3 cases of metastatic carcinoma and metastatic lymph node tissues were positive, but the positive rate was no significant difference; (2) UL30 gene fragment mainly located in the cytoplasm of tumor epithelial cells; (3) The UL30 gene fragment persisted in the tumor epithelial cells in the process of tumor invasion and metastasis; (4) There were no morphological features of HSV Ⅰ proliferative infection in the presence of HSV Ⅰ gene fragment. Tip HSV Ⅰ may be a special way of infection in the occurrence and development of oral squamous cell carcinoma play a role.