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在肺炎支原体(MP)感染中,黏附宿主呼吸道上皮细胞和成功定植是感染的关键一步,黏附通过MP表面的P1蛋白介导与宿主细胞的分子受体结合。传统上基于P1基因序列的变异将MP分为两型,新近研究表明,在P1基因上有2个特异的重复序列,一个重复序列被命名为RepMP4,位于编码区的5′末端,另一个为RepMP2/3,位于3′末端。针对这两个重复序列的PCR扩增,结果用限制性内切酶分析,使分型更为细致,共检出8个亚型,这表明在P1基因型中存在着更多的亚型。P1基因的变异可能通过重复序列之间的重组出现在MP染色体其他的位置上而发生。通过直接测序法对临床分离株P1基因更为广泛的研究可做出更精确的分型,以进一步了解P1基因多态性的现状。
In Mycoplasma pneumoniae (MP) infection, adhesion to host respiratory epithelial cells and successful colonization are key steps in infection, and adhesion through the P1 protein on the MP surface mediates binding to the host cell's molecular receptors. Traditionally, MPs have been divided into two types based on the variation of the P1 gene sequence. Recent studies have shown that there are 2 specific repeats on the P1 gene, one repeats named RepMP4, located at the 5 'end of the coding region and the other is RepMP2 / 3, located at the 3 'end. Aiming at the PCR amplification of these two repeats, the results were analyzed by restriction endonucleases to make the typing more detailed. A total of 8 subtypes were detected, indicating that there are more subtypes in the P1 genotype. Mutations in the P1 gene may occur at other positions on the MP chromosome by recombination between repeat sequences. More accurate typing of the P1 gene of clinical isolates can be made more accurate by direct sequencing to further understand the current status of the P1 gene polymorphism.