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通过对５０株野生型藤仓赤霉菌的筛选，获得一株ＧＡ９组分较高而ＧＡ３、ＧＡ４＋７组分较低的菌株农大２０１（ＮＤ２０１），然后通过多次紫外诱变，使其产量从原来的３４μｇ／ｍｌ提高到２６０μｇ／ｍｌ。当发酵条件采用变温培养（培养７２ｈ后由２８℃转到３４℃）、调节ｐＨ值（７２ｈ后ｐＨ由４５调到６２），产量可达３００μｇ／ｍｌ。在培养基按最佳组分配制后，ＧＡ９的产量可达３５０μｇ／ｍｌ。发酵产物经提取、层析并经气相色谱质谱联机（ＧＣＭＳ）鉴定确证为ＧＡ９。硅胶Ｇ薄层层析和荧光光度法可简便地对ＧＡ９进行定量测定。ＨＰＬＣ法可测得ＧＡ９组分的比例含量
A strain of Nongda 201 (ND201) with higher GA9 and lower GA3 and GA4 + 7 components was obtained by screening 50 wild strain Fujimori Fujifilm. After several UV mutagenesis, its yield From the original 34μg / ml increased to 260μg / ml. When the fermentation conditions were changed to warm culture (from 28 ℃ to 34 ℃ after 72h culture), the pH value was adjusted (pH was adjusted from 4.5 to 62 after 72h) and the yield could reach 300μg / ml. GA9 yields up to 350 μg / ml after the medium is formulated with the best ingredients. Fermentation products were extracted, chromatographed and identified by gas chromatography-mass spectrometry (GC-MS) identified as GA9. Silica G thin layer chromatography and fluorescence spectrophotometry GA9 can be easily quantitatively determined. HPLC method can be measured GA9 component ratio content