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Nitrogen content is an important parameter for petroleum refining processes.The combined use of mid-infrared attenuated total reflection spectroscopy and multivariate calibration allows accurate determination of nitrogen content in petroleum and its products.The calibration models of nitrogen content in crude oils have been established by partial least squares (PLS) method.The results predicted by this method were very close to those determined by standard methods.Compared with standard methods,this method is provided with advantages such as high-speed,simplicity and good-repeatability without any needs for pretreatment.