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此法对确定组织细胞与组织细胞型淋巴瘤效果较好。我们根据Cannon报道的方法加以改良,其步骤如下: 1.取新鲜组织甲醛——蔗糖固定液和Holt氏液处理(各20—24小时),水洗,经冷丙酮(Ⅰ、Ⅱ)脱水各1小时,经冷2甲苯(Ⅰ、Ⅱ)透明各30分钟,浸蜡1—2小时后包埋。 2.常规切片脱蜡至水(从二甲苯→无水丙酮→稀丙酮至水)。 3.入孵育液(不加Melaola蓝)37℃1—3小时。 4.蒸馏水洗时间稍久,以免组织切片上沉积染料影响观察。 5.核固红染液复染1—2分钟。 6.蒸馏水洗→无水酒精脱水烤干→中性树胶封固。结果:良性和肿瘤性组织细胞胞浆内的中性非特异性酯酶呈蓝黑色弥漫阳性,背景和胞核均呈红色。
This method is better for the determination of tissue cells and histiocytic lymphoma. According to the method reported by Cannon, the steps are as follows: 1. Take fresh tissue formaldehyde - sucrose solution and Holt’s solution (20-24 hours each), wash, and dehydrate with cold acetone (I, II). Hours, cold 2 toluene (I, II) transparent for 30 minutes, embedded in wax after 1-2 hours. 2. Conventional sections are deparaffinized to water (from xylene to anhydrous acetone to dilute acetone to water). 3. Incubate (without adding Melaola Blue) for 1 to 3 hours at 37°C. 4. The distilled water wash time is slightly longer to avoid the deposition of dye on the tissue section to affect the observation. 5. Counterstain the nuclear solid red dye for 1-2 minutes. 6. Distilled water → anhydrous alcohol dehydrated dry → neutral gum sealed. RESULTS: Neutral non-specific esterases in the cytoplasm of benign and neoplastic tissues were diffused in blue and black, and both background and nucleus were red.