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目的了解临床尿液标本分离的60株大肠埃希菌消毒剂、灭菌剂耐药基因的存在情况。方法收集2006年1月-2008年10月从住院患者尿液中分离出大肠埃希菌60株,应用K-B纸片扩散法检测细菌对15种抗菌药物的敏感性;应用PCR及序列分析3种消毒剂、灭菌剂耐药基因(qacE△1t、ehA、merA)。结果60株大肠埃希菌除对亚胺培南、哌拉西林/他唑巴坦、头孢西丁、阿米卡星、庆大霉素有较高的敏感率(>70.0%)外,对其他10种抗菌药物的敏感率均较低(<50.0%);60株菌中42株检出qacE△1基因(70.0%),10株检出merA基因(16.7%),全部检出tehA基因,1号菌tehA基因测得序列与美国核酸库(GenBank)已登录的tehA基因序列不同,为新亚型。结论临床尿液标分离的大肠埃希菌70.0%携带qacE△1基因,未来细菌消毒剂的耐药可能是医院感染的主要因素之一。
Objective To understand the existence of 60 Escherichia coli disinfectants and sterilizing agent resistant genes isolated from clinical urine samples. Methods Sixty isolates of Escherichia coli were isolated from the urine of inpatients from January 2006 to October 2008. The susceptibility of bacteria to 15 antibiotics was detected by KB disk diffusion method. Three kinds of antibacterials were tested by PCR and sequence analysis Disinfectant, sterilant resistance gene (qacE △ 1t, ehA, merA). Results 60 strains of Escherichia coli had higher sensitivity (> 70.0%) to imipenem, piperacillin / tazobactam, cefoxitin, amikacin and gentamycin The sensitivity of other 10 kinds of antimicrobial agents were low (<50.0%); 42 strains of 60 strains detected qacE △ 1 gene (70.0%), 10 strains detected merA gene (16.7%), all detected tehA gene The sequence of tehA gene of No. 1 strain is different from the tehA gene sequence registered in GenBank and is a new subtype. Conclusion 70.0% of Escherichia coli isolated from clinical urine samples carry qacE △ 1 gene. The future of bacterial disinfectant drug resistance may be one of the main factors of nosocomial infection.