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目的研究白藜芦醇对人胃癌SGC-7901细胞的影响。方法 SGC-7901细胞体外培养48h,分为白藜芦醇低、中、高剂量组(44、88、176μmol/L),阳性对照组(5-FU153.8μmol/L);阴性对照组(不含药物同体积培养液),荧光显微镜观察不同浓度的白藜芦醇对SGC-7901细胞的形态学影响;流式细胞仪检测白藜芦醇对肿瘤细胞中线粒体膜电位、活性氧的的影响;激光共聚焦显微镜观察白藜芦醇对肿瘤细胞中钙离子浓度的影响。结果显微镜下可见肿瘤细胞染色程度加深,染色质聚集、断裂,产生大小不等的凋亡小体,且随着白藜芦醇浓度加大,现象越来越明显,表明细胞凋亡的比例不断增加;白藜芦醇能够明显降低肿瘤细胞中线粒体膜电位,随着白藜芦醇浓度不断增加,肿瘤细胞中活性氧也不断增加,说明白藜芦醇能够提高肿瘤细胞中的活性氧水平来诱导其凋亡;白藜芦醇对肿瘤细胞中钙离子的浓度有一定的作用,其中高剂量能够显著提高肿瘤细胞中钙离子的浓度,且呈现一定的剂量依赖关系。结论白藜芦醇通过影响SGC-7901肿瘤细胞线粒体膜电位、活性氧及钙离子浓度导致肿瘤细胞凋亡,且与剂量相关。
Objective To study the effect of resveratrol on human gastric cancer cell line SGC-7901. Methods SGC-7901 cells were cultured in vitro for 48h and divided into low, medium and high doses of resveratrol (44,88,176μmol / L) and positive control group (5-FU153.8μmol / L). Negative control group Drug-containing volume of culture medium). The morphological changes of SGC-7901 cells were observed by fluorescence microscopy. The effects of resveratrol on mitochondrial membrane potential and reactive oxygen species in tumor cells were detected by flow cytometry The effect of resveratrol on the concentration of calcium in tumor cells was observed by laser confocal microscopy. Results Under the microscope, the degree of staining of tumor cells deepened, chromatin gathered and ruptured, and apoptotic bodies with different sizes were produced. With the increase of resveratrol concentration, the phenomenon became more and more obvious, indicating that the proportion of apoptotic cells Increased; resveratrol can significantly reduce mitochondrial membrane potential in tumor cells, with the increasing concentration of resveratrol, reactive oxygen species in tumor cells also increased, indicating that resveratrol can increase the level of reactive oxygen species in tumor cells Induced apoptosis. Resveratrol had a certain effect on the concentration of calcium in tumor cells, and the high dose of it could significantly increase the concentration of calcium in tumor cells, and showed a dose-dependent manner. Conclusion Resveratrol induces tumor cell apoptosis by affecting mitochondrial membrane potential, reactive oxygen species and calcium ion concentration in SGC-7901 tumor cells, and is dose-dependent.