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剥取脱毒甘薯只带一叶一心大小的茎尖分生组织接种在ＭＳ＋１．０ｍｇ／Ｌ６ＢＡ固体培养基上，待其成活后，将其转管接种在ＭＳ＋２．０ｍｇ／Ｌ２，４－Ｄ固体培养基上，进行胚性愈伤组织的诱导，挑选状态好的胚性愈伤组织转移到ＭＳ＋２．０ｍｇ／Ｌ２，４－Ｄ的液体中，进行振荡培养，建立胚性细胞悬浮培养系。经过一段时间的悬浮培养，选择其中较大的胚性愈伤组织接种在ＭＳ＋２．０ｍｇ／Ｌ２，４－Ｄ固体培养基上进行胚性愈伤组织的增殖及体胚的诱导，４周后将体胚及愈伤组织转移到ＭＳ＋１．０ｍｇ／ＬＡＢＡ的固体培养基上，诱导形成丛生芽，继而形成小植株，将小植株切下转移到不加任何激素的ＭＳ固体培养基上诱导生根，生长发育成完整植株。通过悬浮培养方法可以在短时间内繁殖大量的脱毒试管苗。
The stripped sweet potato was inoculated on MS + 1.0 mg / L 6BA solid medium with only one leaf-shaped shoot apical meristem. After its survival, it was inoculated into MS + 2.0 mg / L 2,4-D solid The embryogenic callus was induced on the medium, and the embryogenic callus selected well was transferred to the MS + 2.0mg / L, 4-D liquid and cultured in shaking to establish an embryogenic cell suspension culture system. After a period of suspension culture, the larger embryogenic callus was selected and inoculated on MS + 2.0mg / L, 4-D solid medium for embryogenic callus proliferation and somatic embryo induction. After 4 weeks Somatic embryos and callus were transferred to MS + 1.0mg / LABA solid medium to induce the formation of clusters of shoots, which formed the plantlets. The plants were excised and transferred to MS solid medium without any hormones to induce rooting and growth Into a complete plant. Through the suspension culture method can reproduce a large number of virus-free tube seedlings in a short time.