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为延续不易获得的高度不实的大小麦杂种,试行了以杂种F_1和BC_1幼穗切段为外植体的组织培养无性繁殖。当诱导培养基选用N_6+2,4—D2毫克/升时,愈伤组织平均诱导率为82.4％。在杂种F_1幼穗培养中,21.1％的愈伤组织直接在上述培养基上产生了芽丛。分化培养基试用了不含激素的MS和Ns培养基,杂种BCl幼穗愈伤组织在MS上分化率为8.82％,在N_6上未见有分化。已从一个杂种F_1幼穗培养中获得了45株再生植株,对其中6株根尖细胞学观察表明,5株体细胞染色体数为27条,一株为26条,分别比原外植体体细胞染色体数28少一至二条。杂种BC_1幼穗再生植株正在培养中。
In order to extend the highly inaccurate large and hard wheat hybrids which were hard to be obtained, a trial of clonal propagation of tissue culture with the hybrid F1 and BC1 young cuttings as explants was conducted. The average induction rate of callus was 82.4% when N 6 + 2,4-D2 mg / L was used as induction medium. In hybrid F 1 panicle culture, 21.1% of the callus produced shoots directly on the above medium. The hormone-free MS and Ns medium were used for the differentiation medium. The differentiation rate of the hybrid callus of young BCl on MS was 8.82% and no differentiation on N_6. A total of 45 regenerated plants were obtained from a hybrid F_1 panicle culture. Among them, 6 root tips showed that the chromosome number of 5 somatic cells was 27 and that of a strain was 26, which were respectively higher than that of the original explant Cell chromosome number 28 from one to two. Hybrid BC_1 young spike regenerated plants are under cultivation.