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本研究将经过高浓度生长素预培养的剑麻愈伤组织细胞.接种入LS、MS、N_6、B_5四种液体培养基中,在30d培养过程中动态每2-5d测定培养细胞的生长速率、剑麻蛋白酶活力和培养液的pH值变化。结果表明:悬浮培养细胞的生长量成倍地高于固体培养细胞;LS、MS培养基是剑麻细胞悬浮培养及诱导产蛋白酶最适的培养基;悬浮培养液中的蛋白酶活力占细胞蛋白酶活力的20.0％-46.9％;悬浮培养细胞生长速率与培养液的pH值存在一定的相关性。
In this study, the callus tissue of Sisal hemp pre-cultured with high concentration of auxin was inoculated into four liquid mediums of LS, MS, N_6 and B_5, and the growth rate of the cultured cells was determined every 2-5 days during 30 days of culture , Sisal protease activity and pH of the culture medium. The results showed that the growth of suspension cultured cells was doubled that of solid cultured cells. LS and MS medium were the optimal culture medium for sisal suspension culture and induced protease production. The activity of protease in suspension medium accounted for the activity of cell protease Of the 20.0% -46.9%; the growth rate of suspension culture and the pH of the culture medium there is a certain correlation.