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利用蛋白酶解法从完整抗体制备 Fv 片段是个费时费力的繁琐过程,且对于多种McAb 的 Fv 片段制备是不成功的。利用 PCR 方法,从抗体产生细胞中获得可变区基因,并利用基因工程技术,构建不同的目的基因:重链可变区(VH)和轻链可变区(VL)相连构成 Fv,VH 和VL 连入一基因编码的连接肽(Linker)构成 scFv,两个 scFv 相连构成双价 scFv,两种功能 scFv相连构成双功能 scFv 等。本文就 Fv 抗体及其衍生物基因的体外构建作一综述。
The preparation of Fv fragments from intact antibodies using proteolytic methods is a cumbersome and laborious process and is unsuccessful for Fv fragment preparation of a variety of McAbs. The variable region genes were obtained from antibody-producing cells by PCR and gene engineering was used to construct different target genes: the heavy chain variable region (VH) and the light chain variable region (VL) were linked to form the Fv, VH and Linkage of VL into a gene encoding linker (Linker) to form scFv, two scFv connected to form a bivalent scFv, two functional scFv connected to form a dual function scFv and so on. This review summarizes the in vitro construction of the Fv antibody and its derivatives.