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以甜樱桃’拉宾斯’(Prunus avium L.’Lapins’)为试材,采用RT-PCR结合RACE技术,克隆获得1个与花发育相关的MADS box基因,命名为PaMADS3,其在GenBank中的登录号为HQ229605。PaMADS3基因全长1095bp,包含1个723bp的开放阅读框,推断其编码240个氨基酸。序列分析表明:PaMADS3蛋白与拟南芥中的SEP蛋白高度同源。组织特异性表达显示PaMADS3基因在花瓣、雄蕊、心皮中表达。实时定量RT-PCR分析表明,花芽露绿期的离体枝条经过15和25℃处理后,其雌蕊中PaMADS3基因在25℃的表达量高于在15℃的表达量。
Using Prunus avium L.’Lapins’ as test material, one MADS box gene related to flower development was cloned by RT-PCR and RACE technology and named as PaMADS3, which was cloned in GenBank The accession number is HQ229605. PaMADS3 gene is 1095bp in length and contains a 723bp open reading frame, which deduced that it encodes 240 amino acids. Sequence analysis showed that the PaMADS3 protein was highly homologous to the SEP protein in Arabidopsis thaliana. Tissue-specific expression of the PaMADS3 gene was observed in petals, stamens, and carpels. Real-time quantitative RT-PCR analysis showed that the expression level of PaMADS3 gene in pistil at 25 ℃ was higher than that at 15 ℃ after treated with 15 and 25 ℃ in vitro.