论文部分内容阅读
肌动蛋白微丝是血管内皮细胞抵抗血流切应力作用,维持内皮单层结构完整的重要粘附结构,是内皮细胞生物力学特性的超微结构基础.本研究应用共聚焦激光扫描显微系统(Leica公司)观察了体外培养的牛主动脉内皮细胞汇聚前、后细胞内肌动蛋白微丝的动态分布、肌动蛋白微丝用特异性荧光探针Rhodamine—Phalloidin(Molecular ProbesInc)染色.用共聚焦激光扫描显微系统对荧光染色的牛主动脉内皮系统断层扫描后,显示牛主动脉
Actin microfilament is a vascular endothelial cell shear stress against blood flow to maintain the integrity of the endothelial monolayer structure of the important adhesion structure is the ultrastructure of endothelial cell biomechanical characteristics of the microstructure of the study using confocal laser scanning microscopy (Leica) observed the dynamic distribution of intracellular actin microfilaments before and after the in vitro culture of bovine aortic endothelial cells, and actin microfilaments were stained with the specific fluorescent probe Rhodamine-Phalloidin (Molecular Probes Inc.) Confocal laser scanning microscopy of fluorescently-stained bovine aortic endothelium showed that the bovine aorta