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花药培养,是小麦育种的一种新技术。提高愈伤组织诱导频率和绿苗分化成活率,是花粉培养的关键技术问题。1980年我们应用合成培养基和简化培养基进行培养;用增产灵对接种前的穗子进行予处理;在诱导培养中,进行了照光和黑暗两种处理,明确了诱导效果。在绿苗移栽方面,初步明确了提高绿苗成活率的土壤条件。材料和方法供试材料为我所的小麦杂种一代,共21个组合。接种时的花粉发育阶段为单核中、晚期。接种前先用70％的酒精擦洗穗鞘,剥去穗鞘后,将各个组合分别扎成小捆浸入0.1％的升汞溶液中7—8分钟,用灭菌水冲2—3次,在无菌条件下接种,然后置于
Anther culture is a new technique of wheat breeding. Increasing callus induction frequency and green plantlet differentiation survival rate are the key technical problems of pollen culture. In 1980, we used synthetic medium and simplified culture medium to cultivate; before spiking with Zaibai Ling pretreatment; in the induction culture, the two light and dark treatment, a clear induction effect. In the green seedlings transplanting, preliminary clear to improve the survival rate of green plant soil conditions. MATERIALS AND METHODS The materials tested were wheat hybrids of our generation with 21 combinations. The stage of pollen development during inoculation is mononuclear and late. Scrub the ear sheath with 70% alcohol before inoculation, stripping the ear sheath, the various combinations were tied into small bundles of mercury immersed in 0.1% solution of mercury 7-8 minutes, washed 2-3 times with sterile water in the Aseptically inoculated, then placed