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丝裂霉素C是一种广谱抗肿瘤抗生素,对多种癌症有抗癌作用,其作用原理可使细胞的DNA发生链间交联,引起DNA双链断裂,阻碍DNA的复制,从而抑制肿瘤细胞分裂。临床上主要用于胃癌、肠癌、肝癌及胰腺癌等消化道癌方面的治疗。本文研究丝裂霉素C对转染人BLM解旋酶基因(shRNA载体)前后前列腺癌PC3细胞活性的影响。使用前期成功构建的干扰载体转染PC3细胞,在转染48 h后加药,通过荧光定量PCR、MTT法、Transwell小室实验、细胞划痕实验、流式细胞术,分别检测加药12、24、36 h BLM基因的表达量、PC3细胞增殖能力、侵袭能力、迁移能力及凋亡情况的变化。结果显示,敲减BLM基因表达后的PC3细胞相对于正常PC3细胞其增殖能力、侵袭能力和迁移能力能显著被丝裂霉素C抑制,且丝裂霉素C能显著促进其细胞的凋亡,说明BLM基因低表达的前列腺癌细胞对丝裂霉素C更敏感。研究结果为丝裂霉素C在前列腺癌的临床治疗上奠定了理论基础。
Mitomycin C is a broad-spectrum anti-tumor antibiotics, anti-cancer effect on a variety of cancers, the principle of its role in the cell DNA can occur between chains cross-linked, causing DNA double-strand breaks, hinder DNA replication, thereby inhibiting Tumor cell division. Clinically for gastric cancer, colon cancer, liver cancer and pancreatic cancer and other gastrointestinal cancer treatment. This study was designed to investigate the effect of mitomycin C on the activity of PC3 cells before and after transfected with human BLM helicase gene (shRNA vector). PC3 cells were transfected with the interference vectors successfully constructed in the previous period, and then dosed 48 h after transfection. The fluorescence quantitative PCR, MTT assay, Transwell chamber assay, cell scratch assay and flow cytometry were used to detect the dosing of PC3 cells. , 36 h BLM gene expression, PC3 cell proliferation, invasion, migration and apoptosis changes. The results showed that the proliferation, invasion and migration of PC3 cells knocked out by BLM gene expression were significantly inhibited by mitomycin C compared with that of normal PC3 cells, and mitomycin C could significantly promote the apoptosis of PC3 cells , Indicating that BLM gene low expression of prostate cancer cells more sensitive to mitomycin C. The results of mitomycin C in the clinical treatment of prostate cancer laid the theoretical foundation.