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本实验旨在探索体外细胞免疫的可行性 ,即脾淋巴细胞与卡介苗在体外培养条件下能否使淋巴细胞致敏 ,产生抗结核特异性转移因子 (STFTB)。方法 :采用狗脾淋巴细胞与卡介苗共同孵育培养 ,收集培养后的细胞 ,用超声粉碎法破碎细胞 ,中空纤维超滤器超滤 ,收集超滤液即为狗脾抗结核特异性转移因子 (CS -STFTB)。对该制剂进行理化指标及相关的免疫学试验。结果 :该制剂为淡黄色澄清黄色澄清透明液体 ,多肽含量 1.0 3mg/ml,核糖含量 87μg/ml。蛋白质反应 (- )。紫外最大吸收峰在 2 5 8nm ,E2 6 0nm/ 2 80nm为2 .6 6 7。CS -STFTB与白细胞、PPD共同孵育数减低。能够拮抗HC引起的吞噬功能下降。结论 :用体外免疫法制备的CS -STFTB符合转移因子制检标准 ,可提高机体的特异性和非特异性免疫功能。
The purpose of this experiment is to explore the feasibility of in vitro cellular immunity, that is, splenic lymphocytes and BCG could sensitize lymphocytes in vitro and produce anti-TB-specific transfer factor (STFTB). Methods: The spleen lymphocytes were incubated with Bacillus Calmette-Guerin (BCG) and cultured. The cultured cells were harvested and the cells were disrupted by sonication. Hollow fiber ultrafilter was ultrafiltrated to collect the ultrafiltrate. -STFTB). The preparation of physical and chemical indicators and related immunological tests. Results: The preparation was light yellow clear yellow transparent liquid. The polypeptide content was 1.0 3mg / ml and the ribose content was 87μg / ml. Protein reaction (-). UV maximum absorption peak at 258nm, E2 6 0nm / 2 80nm to 2.667. CS-STFTB co-incubation with leukocytes, PPD decreased. Can antagonize HC-induced phagocytic decline. CONCLUSIONS: CS-STFTB prepared by in vitro immunostaining meets the criteria for the production of transfer factor and enhances the specificity and non-specific immune function.