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为确定结核样型和瘤型麻风病人的血液单核细胞处理细胞内微生物的能力是否存在缺陷,作者按Lehrer介绍的姬姆萨染色白细胞杀死试验的改良法,从9例极型瘤型(LL)、8例极型结核样型(TT)麻风病人和11名正常人对照者的外周血中分离单一核细胞,制备单核细胞的附着细胞单层,经与伪热带念珠菌(能被缺乏髓过氧化物酶活性的成熟的单核细胞溶解)分别孵育30和60分钟后,计数每100个单核细胞所摄入的念珠菌的平均数和每
To determine whether the ability of blood mononuclear cells of tuberculoid and leprous leprosy cells to process intracellular microbes is flawed, the authors modified the Giemsa-stained Leukocyte Killing Assay described by Lehrer, LL mononuclear cells were isolated from the peripheral blood of 8 patients with leprosy of tuberculous type (TT) and 11 normal controls to form monolayers of mononuclear cells adhered to Candida tropicalis Mature mononuclear cells lacking myeloperoxidase activity) were incubated for 30 and 60 minutes, respectively, and the average number of candida ingested per 100 monocytes was counted and each