论文部分内容阅读
目的:探讨肝细胞凋亡在肝细胞癌发生和发展中所起的作用及其与 HBV 感染的关系。方法:用免疫组织化学方法检测55例肝细胞癌患者的肝癌和癌旁肝组织中的 HBsAg、HBcAg、Fas、FasL,同时用 Tunel 反应原位检测细胞死亡。结果:55例肝癌组织中,41例 HBsAg 阳性,其中25例 HBcAg 阳性;42例可见部分浸润的淋巴细胞中有 Fas 抗原表达,信号较强;癌旁肝组织中 Fas 抗原阳性者23例,肝癌组织中8例,阳性信号均较弱,FasL 在局部浸润的淋巴细胞中检出38例,肝癌及癌旁组织均为阴性。Tunel 反应在3例阳性对照的肝炎标本中呈强阳性反应,肝癌组织中未发现有明显的阳性信号,但有18例标本在浸润的淋巴细胞中检出阳性结果。结论:细胞凋亡机制在 HCC 发生和发展过程中起重要作用。
Objective: To explore the role of hepatocyte apoptosis in the occurrence and development of hepatocellular carcinoma and its relationship with HBV infection. METHODS: HBsAg, HBcAg, Fas, and FasL were detected by immunohistochemistry in 55 cases of hepatocellular carcinoma (HCC) and hepatoma tissues. The cell death was detected in situ using the Tunel reaction. RESULTS: Among the 55 cases of HCC, 41 cases were positive for HBsAg, of which 25 cases were positive for HBcAg; 42 cases showed partially expressed infiltrating lymphocytes with Fas antigen expression and strong signals; 23 cases with Fas antigen positive in adjacent liver tissues. In 8 cases of tissues, positive signals were weak, and 38 cases of FasL were detected in locally infiltrated lymphocytes, and liver cancer and adjacent tissues were negative. The Tunel reaction was strongly positive in 3 positive control hepatitis specimens. No significant positive signal was found in the liver cancer tissue, but 18 specimens detected positive results in infiltrating lymphocytes. Conclusion: The mechanism of apoptosis plays an important role in the occurrence and development of HCC.